Voltammetric studies on the interaction of ds-DNA with anti-cancer agents imatinib and erlotinib - Comparison of Au and Pt nanoparticle effects to drug-DNA complex formation

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Eskiköy Bayraktepe D., Yıldız C., Yazan Z.

JOURNAL OF MOLECULAR LIQUIDS, cilt.388, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 388
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1016/j.molliq.2023.122824
  • Dergi Adı: JOURNAL OF MOLECULAR LIQUIDS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chemical Abstracts Core, Chimica, Compendex, INSPEC
  • Anahtar Kelimeler: Bimetallic nanoparticles, DNA interaction, Erlotinib, Imatinib, Screen printed electrode, Voltammetry
  • Ankara Üniversitesi Adresli: Evet

Özet

The interactions of imatinib (IMA) and erlotinib (ERL) with calf-thymus double-stranded deoxyribonucleic acid (ds-DNA) were studied by using monometallic (Au and Pt) and bimetallic (Au + Pt) nanoparticles modified DNA biosensors. The prepared electrochemical biosensors were designed to show the interaction between IMA (or ERL) and ds-DNA. The characterization of the prepared sensors was carried out by energy-dispersive X-ray spectroscopy (EDX). The influence of Au, Pt, and Au + Pt nanoparticles in the modified biosensor was investigated to elucidate the interaction between the anti-cancer drugs and ds-DNA. The interaction was realized based on the electrochemical oxidation signal (at about +0.80 V) of the guanine (dGu) base of ds-DNA by differential pulse voltammetry (DPV) in pH 4.8 acetate buffer. The effect of the nanoparticle's concentration and interaction time of IMA (and ERL) with ds-DNA on the electrochemical response of dGu were optimized. The results showed that Au + Pt nanoparticles, especially for IMA, significantly affected the binding constant value of the drugs to ds-DNA. Interaction studies have also shown that the binding mode for IMA and ERL could be intercalation and groove binding and the binding constant values were found to be 68.3 x 106 M-1 and 27.3 x 107 M-1, respectively.