Göktaş H., Tüfekçi M., Demir Çalışkan T., Özgüzar H. F., Göçmen J. S., Evren Yurtcu E., ...Daha Fazla
41st World Congress of Endourology and Urotechnology WCET 2024, Seoul, Güney Kore, 12 - 16 Ağustos 2024, cilt.38, sa.38, ss.33, (Özet Bildiri)
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Yayın Türü:
Bildiri / Özet Bildiri
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Cilt numarası:
38
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Basıldığı Şehir:
Seoul
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Basıldığı Ülke:
Güney Kore
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Sayfa Sayıları:
ss.33
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Ankara Üniversitesi Adresli:
Evet
Özet
Introduction: Hospital-acquired infections (HAI) are widely known infection related to widespread use of medical devices. Catheter-associated urinary tract infections (CAUTI) are one of the most challenging complications among HAIs in short- and long-term catheterization. Antifouling strategies have been developed in order to eliminate these complications. The goal of antifouling strategies is to prevent the tendency of bacteria to adhere to the surface of the relevant medical device. The aim of the study is to prevent bacterial colonization in catheters by producing a non-adherent surface for Escherichia coli bacteria. Gram- negative bacteria cause approximately 95% of CAUTI. E. coli is a gram-negative bacterium, accounting for approximately 60% of bacteria in urine and affecting human health through biofilm formation.
Methods: In order to repel E. coli (ATCC 25922) bacteria from the surface, copolymer thin films were synthesized using polyethylene glycol (PEG) with proven antifouling properties, citric acid (CA) with antimicrobial properties, and glycidyl methacrylate (GMA) used as a binding agent. Functional PEG-GMA- CA thin films at nm level were deposited on the catheter surface by plasma enhanced chemical vapour deposition (PECVD) technique. FTIR, XPS, SEM and contact angle techniques were used to characterize the thin films.
Results: With the characterization methods, it has been observed that the structures of the materials are preserved, and thin film synthesis were carried out without causing changes in the chemical and molecular natures of the materials. The results obtained from microbiology studies show that biofilm formation was prevented by 99.4% even at the end of 30 days compared to uncoated catheters.
Conclusions: This study is the first to investigate the antifouling properties of PEG-GMA-CA thin films produced by PECVD technique. In addition to preventing bacterial colonization for 30 days, the thin film coated catheters are planned to be used in vivo studies due to their inhibition of protein adsorption.
Funding: The study was granted by THE SCIENTIFIC AND TECHNOLOGICAL RESEARCH COUNCIL OF TÜRKİYE.