Akademik Veri Yönetim Sistemi
VETERINARSKI ARHIV, cilt.85, sa.1, ss.59-65, 2015 (SCI-Expanded)
Diagnosis of staphylococcal mastitis cases may sometimes prove problematic due to the nature of the bacterium. Thus, in the present study, the effect of freezing and thawing bovine milk samples on the performance of standard cultures of staphylococcal mastitis was investigated to improve the diagnosis. Each of a total of 228 quarter milk samples from clinical and subclinical mastitis cases was plated on 7 A sheep blood agar directly, and after first being subjected to a process of freezing and thawing. The culture results from two methods were compared on the basis of Staphylococcus spp. positive udder counts and alteration in colony counts of the strains. In the first method. Staphylococcus spp. were isolated from 91 milk samples. In the second method, Staphylococcus spp. were isolated from an additional 11 milk samples that were negative in the first method. Staphylococcus spp. positive udder counts were found lobe significantly different between the two methods. The changes in colony counts of the strains between the two methods were also found to be statistically significant in both clinical and subclinical mastitis cases, with the second method performing better. Consequently, these results indicate that a simple preculture step consisting of freezing and thawing milk samples has advantages for more sensitive diagnosis of staphylococcal mastitis in cattle milk.