Detection of Reactive Oxygen and Nitrogen Species by Electron Paramagnetic Resonance (EPR) Technique

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SÜZEN S., GÜRER ORHAN H., Saso L.

MOLECULES, cilt.22, sa.1, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 22 Sayı: 1
  • Basım Tarihi: 2017
  • Doi Numarası: 10.3390/molecules22010181
  • Dergi Adı: MOLECULES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: free radical, oxidative stress, EPR, ROS, RNS, NITRIC-OXIDE SYNTHASE, RAPID-SCAN EPR, IN-VIVO, OXIDATIVE STRESS, SUPEROXIDE, SPECTROSCOPY, CELLS, NO, STABILITY, MELATONIN
  • Ankara Üniversitesi Adresli: Evet

Özet

During the last decade there has been growing interest in physical-chemical oxidation processes and the behavior of free radicals in living systems. Radicals are known as intermediate species in a variety of biochemical reactions. Numerous techniques, assays and biomarkers have been used to measure reactive oxygen and nitrogen species (ROS and RNS), and to examine oxidative stress. However, many of these assays are not entirely satisfactory or are used inappropriately. The purpose of this chapter is to review current EPR (Electron Paramagnetic Resonance) spectroscopy methods for measuring ROS, RNS, and their secondary products, and to discuss the strengths and limitations of specific methodological approaches.