Polymorphisms of microsomal epoxide hydrolase and glutathione S-transferase P1 in a male Turkish population

Ada A. O., SÜZEN H. S., Iscan M.

INTERNATIONAL JOURNAL OF TOXICOLOGY, cilt.26, sa.1, ss.41-46, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 1
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1080/10915810601118222
  • Dergi Adı: INTERNATIONAL JOURNAL OF TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.41-46
  • Anahtar Kelimeler: EPHX1, GSTP1, polymorphism, Turkish population, GENETIC POLYMORPHISMS, LUNG-CANCER, COLORECTAL-CANCER, BIOTRANSFORMATION ENZYMES, BLADDER-CANCER, NULL-GENOTYPE, BLOOD-CELLS, RISK, ASSOCIATION, SUSCEPTIBILITY
  • Ankara Üniversitesi Adresli: Evet

Özet

Polymorphic genes encoding drug- metabolizing enzymes may account for interindividual differences in certain types of diseases especially cancer. In this study, microsomal epoxide hydrolase ( EPHX1) and glutathione S-transferase P1 ( GSTP1) gene polymorphisms were determined among 133 healthy males of a Turkish population. Frequencies of EPHX1 and GSTP1 gene polymorphisms were determined by using the polymerase chain reaction - restriction fragment length polymorphism ( PCR/ RFLP) method. The observed genotype frequencies of EPHX1 exon 3 were Tyr113Tyr: 50.4%, Tyr113His: 42.1%, His113His: 7.5% and EPHX1 exon 4 were His139His: 69.2%, His139Arg: 28.6%, Arg133Arg: 2.2%. GSTP1 exon 5 genotype frequencies were Ile105Ile: 58.7%, Ile105Val: 35.3%, Val105Val: 6.0% and GSTP1 exon 6 genotype frequencies were Ala114Ala: 85.0%, Ala114Val: 14.3%, Val114Val: 0.7%. These results reveal that the frequencies of EPHX1 and GSTP1gene polymorphisms in a small sampling of males within a Turkish population are similar to European Caucasian populations.