Evaluation of Free Radical Scavenging, Anti-inflammatory and Hypoglycemic Activity of Helianthemum oelandicum subsp. incanum (Willk.) G. Lopez from Türkiye

AĞCA A. C., YILMAZ SARIALTIN S., YILMAZ B. S.

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, cilt.26, sa.2, ss.227-233, 2023 (TRDizin)

Özet

Helianthemum is represented by 19 taxa, and out of four are endemic in Türkiye. The antioxidant, anti-inflammatory, and hypoglycemic activities of the ethanol (75%) and aqueous extracts from aerial parts of Helianthemum oelandicum subsp. incanum growing in Türkiye was examined in this study. The water extract exhibited antioxidant activity with 67.26±0.49 and 68.08±0.41 μg ml-1 values on DPPH and ABTS radicals, respectively. The antioxidant activity value of ethanol extract on DPPH radical was found as 46.71±0.27 μg ml-1, where as this value was determined as 51.60±0.76 μg ml-1 for scavenging effects on ABTS radical. Both ethanol and aqueous extracts possessed in vitro anti- inflammatory activity by inhibiting erythrocyte membrane hemolysis in a concentration dependent manner and ethanol extracts exhibited greater activity than aqueous extracts. By measuring the inhibitory effect of the extracts on α-glucosidase, in vitro hypoglycemic activity was assessed. According to the results of this study, the IC50 values of both ethanolic and aqueous extracts were found to be close to each other, 2.52±0.01 and 3.21±0.01 μg ml-1, respectively and compared with the standard compound acarbose (IC50= 0.90±0.01 μg ml-1) it was determined that both extracts exhibited strong inhibition on α- glucosidase. Based on all results evaluated, the ethanolic extract displayed higher antioxidant, anti-inflammatory, and hypoglycemic activities than the aqueous extract.