Purification and mass spectrometry based characterization of a pediocin produced by <i>Pediococcus acidilactici</i> 13

Altuntas E., Ayhan K., Peker S., Ayhan B., Demiralp D. O.

MOLECULAR BIOLOGY REPORTS, cilt.41, sa.10, ss.6879-6885, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41 Sayı: 10
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1007/s11033-014-3573-z
  • Dergi Adı: MOLECULAR BIOLOGY REPORTS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.6879-6885
  • Anahtar Kelimeler: Pediococcus acidilactici, Pediocin, Ultrafiltration purification, Proteomic analysis, 2D-PAGE, MALDI-TOF MS, ANTIMICROBIAL ACTIVITY, GENETIC-CHARACTERIZATION, BACTERIOCIN, STRAIN, GROWTH, FERMENTATION
  • Ankara Üniversitesi Adresli: Evet

Özet

Bacteriocins are antimicrobial peptides produced by several bacterial species. Among the bacteriocins pediocin-like bacteriocins have a significant inhibitory activity on the foodborne pathogens especially on Listeria monocytogenes. This study aims to select a simple and usable purification method to purify/concentrate the antimicrobial peptide and characterization of the bacteriocin produced by Pediococcus acidilactici 13 by using proteomic approaches which is a recent omic technology. For purification dialysis, ultrafiltration method was used, and as a result of this study the bacteriocin activity reached 819,200 AU/mL from 102,400 AU/mL initially. Two dimensional gel electrophoresis and then matrix-assisted laser desorption ionization/time of flight mass spectrometry (MALDI-TOF MS) analysis were carried out to identify the current bacteriocin and related proteins. Obtained data revealed similarity to pediocin PA-1 transport/processing ATP-binding protein PedD (accession number: P36497), pediocin operon PedC (accession number: Q68GC4) and bacteriocin pediocin PA-1 (accession number: P29430) from UniProtKB/Swiss-Prot databank, thus the bacteriocin produced by P. acidilactici 13 is considered similar to pediocin PA-1.