Differentiation and molecular characterization of endothelial progenitor and vascular smooth muscle cells from induced pluripotent stem cells

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DASTOURİ M., ÖZDAĞ SEVGİLİ H., AKAR A. R., CAN A.

BIOIMPACTS, cilt.13, sa.4, ss.289-300, 2023 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 13 Sayı: 4
  • Basım Tarihi: 2023
  • Doi Numarası: 10.34172/bi.2022.24132
  • Dergi Adı: BIOIMPACTS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, Directory of Open Access Journals
  • Sayfa Sayıları: ss.289-300
  • Anahtar Kelimeler: Induced pluripotent stem cells, Endothelial progenitor cells, Vascular smooth muscle cells, Lateral mesoderm cell, In vitro differentiation, VASCULOGENESIS, ANGIOGENESIS, RECRUITMENT
  • Ankara Üniversitesi Adresli: Evet

Özet

Introduction: Pluripotent stem cells have been used by various researchers to differentiate and characterize endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) for the clinical treatment of vascular injuries. Studies continue to differentiate and characterize the cells with higher vascularization potential and low risk of malignant transformation to the recipient. Unlike previous studies, this research aimed to differentiate induced pluripotent stem (iPS) cells into endothelial progenitor cells (EPCs) and VSMCs using a step-wise technique. This was achieved by elucidating the spatio-temporal expressions of the stage-specific genes and proteins during the differentiation process. The presence of highly expressed oncogenes in iPS cells was also investigated during the differentiation period.