Analysis of 7,12-dimethylbenz[a]anthracene induced mutations by the use of restriction site mutation technique in the rat

SÜZEN H. S., Parry J.

Fabad Journal of Pharmaceutical Sciences, cilt.24, sa.2, ss.67-73, 1999 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 24 Sayı: 2
  • Basım Tarihi: 1999
  • Dergi Adı: Fabad Journal of Pharmaceutical Sciences
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.67-73
  • Anahtar Kelimeler: DMBA, p53, ras, Restriction site mutation assay
  • Ankara Üniversitesi Adresli: Evet

Özet

The restriction site mutation (RSM) technique was applied as a methodology for the detection of base changes in restriction enzyme recognition sequences. Rats were exposed to 7,12-dimethylbenz[a]anthracene (DMBA) in two different doses. Mutations were analyzed in a variety of restriction enzyme recognition sequences of the rat p53 and ras genes. Mutations were detected in the BslI recognition sequences (CCTGTCCTGGG) in the high dose DMBA treated rats. DNA sequences analyses revealed that the induction of mutation was G→A transition at the tenth base of the CCTGTCCTGGG sequences. The developed method allows the detection of base pair substitutions, small insertions or deletions within the restriction enzyme recognition site in any gene for which the DNA sequence is known without selection of mutant phenotype.