Development of high frequency multiple shoot formation in Persian clover (Trifolium resupinatum L.)

Uranbey S., Sevimay C. S., ÖZCAN S.

PLANT CELL TISSUE AND ORGAN CULTURE, cilt.80, sa.2, ss.229-232, 2005 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 80 Sayı: 2
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1007/s11240-004-0568-9
  • Dergi Adı: PLANT CELL TISSUE AND ORGAN CULTURE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.229-232
  • Anahtar Kelimeler: micropropagation, rooting, Trifolium resupinatum, REGENERATION, SAINFOIN, PLANT
  • Ankara Üniversitesi Adresli: Evet

Özet

An efficient and reliable micropropagation system for Persian clover (Trifolium resupinatunt L.) was developed using different explants and media. Node, hypocotyl and cotyledonary node explants were cultured on Murashige and Skoog (MS) medium supplemented with combinations of either 6-benzyladenine (BA) and indole-3-butyric acid (IBA) or BA, Kinetin (KIN) and IBA. Direct multiple shoots developed within 6 weeks in all explants in most media tested. The best shoot multiplication capacity was obtained from cotyledonary node explants on MS medium containing 7.1 muM BA and I muM IBA or 14.1 muM BA and I muM IBA. Elongated shoots were rooted on either MS medium alone or combination with different concentrations of indole-3-butyric acid (IBA), indole-3-acetic acid (IAA) and alpha-naphthaleneacetic acid (NAA). High rooting was achieved in half strength MS medium containing 8 muM IBA.