High Performance Liquid Chromatographic Analysis of some Diterpenoids of the Ballota species

Sever Yılmaz B., Çitoǧlu G. S.

Fabad Journal of Pharmaceutical Sciences, cilt.28, sa.1, ss.13-17, 2003 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 1
  • Basım Tarihi: 2003
  • Dergi Adı: Fabad Journal of Pharmaceutical Sciences
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.13-17
  • Anahtar Kelimeler: Ballota, Diterpenoids, HPLC analysis, Lamiaceae
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study, the diterpenoids, namely, hispanolone, ballonigrine and dehydrohispanolone, in 16 Ballota taxa growing in Turkey have been determined qualitatively by High-performance Liquid Chromatography (HPLC). Analysis was performed on a normal phase column (Zorbax-CN, 5 μm, 250 × 4.6 mm i.d.). The solvent system consisted of n-Hexane-MeOH (98:2, speed gradient). Peaks were detected at 190-360 nm using Photo-diode array (PDA) dedector. The extracts of Ballota acetabulosa, B. pseudodictamnus subsp. lycia, B. cristata, B. inaequidens, B. saxatilis subsp. saxatilis, B. saxatilis subsp. brachyodonta, B. larendana, B. latibracteolata, B. rotundifolia, B. macrodonta, B. nigra subsp. foetida, B. nigra subsp. uncinata and B. antalyense were found to contain the diterpenoids.