Determination and phylogenetic analysis of canine distemper virus in dogs with nervous symptoms in turkey
ACTA VETERINARIA HUNGARICA, cilt.52, sa.1, ss.125-132, 2004 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 52 Sayı: 1
- Basım Tarihi: 2004
- Doi Numarası: 10.1556/avet.52.2004.1.12
- Dergi Adı: ACTA VETERINARIA HUNGARICA
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.125-132
- Anahtar Kelimeler: canine distemper, nervous symptoms, RT-PCR, dot-ELISA, SYSTEM, BLOOD, RNA
- Ankara Üniversitesi Adresli: Evet
Özet
In the present study, canine distemper virus (CDV) was investigated in 20 dogs having nervous signs arousing the clinical suspicion of canine distemper (CD). A total of 13 animals (65%) were stray dogs and had no accurate record about the vaccination history. Clinical examinations revealed that the majority (85%) of the animals showed systemic form characterised by predominantly nervous symptoms accompanied by mild respiratory system signs whilst the remaining cases (15%) recorded mainly respiratory distress. CDV RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) only in 45% of the suspected cases. Phylogenetic analysis of partial nucleotide sequence of the P gene coding region revealed that the virus is closely related to European strains. Immune responses in 13 cases (65%), which were detected by dot-ELISA, indicated inefficient levels for neutralising functions against CDV. It was postulated that this response could have been mediated by either previous vaccination or mild infection with field strains.