Effect of Homo- and Heterofermentative Silage Additives on the Quality and In Vitro Rumen Digestibility of Two Different Triticale Cultivars
Fermentation, cilt.12, sa.9, ss.417, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 12 Sayı: 9
- Basım Tarihi: 2026
- Doi Numarası: 10.3390/fermentation12090417
- Dergi Adı: Fermentation
- Derginin Tarandığı İndeksler: Food Science & Technology Abstracts, Natural Science Collection (ProQuest), Biological Science Database (ProQuest), Scopus, Science Citation Index Expanded (SCI-EXPANDED), BIOSIS, INSPEC, Directory of Open Access Journals
- Sayfa Sayıları: ss.417
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Ankara Üniversitesi Adresli: Evet
Özet
Triticale, a hybrid cereal crop derived from the
cross of wheat and rye, is widely used for
silage production owing to its high nutritional qualities and tolerance to
varying climatic
conditions. The genotype and maturity stage of forage crops govern the
efficiency of micro-
bial inoculants used for ensiling. The effects of homofermentative (HMF;
Lactiplantibacillus
plantarum and Enterococcus faecium) and heterofermentative (HTF;
Lentilactobacillus buchneri)
inoculants, either alone or in combination, on the fermentation quality,
nutrient compo-
sition, and in vitro rumen digestibility of ensiled triticale varieties
(Alperbey and Karma
2000) were assessed in this study. The study was carried out using a 2 × 2 × 2
factorial
arrangement of two triticale varieties, two levels of HMF [0 or 1 × 105 CFU/g
fresh forage
(0.8 mg/kg)], and two levels of HTF [0 or 0.5 × 105 CFU/g fresh forage (500
mg/kg)].
Forages were harvested at the early dough stage, vacuum-packed in 110 μm-thick
plastic
bags (35 cm × 25 cm), and allowed to ferment for 120 days. Fermentation
characteristics,
chemical composition, volatile fatty acids, and digestibility traits were
evaluated after
ensiling. Application of HMF inoculant significantly reduced silage pH compared
to the
control and HTF treatments in Alperbey, whereas in Karma 2000, terminal pH
remained
statistically similar between control and HMF treatments. Generally, inoculated
silages had
lower ammonia nitrogen levels than control silages, reflecting better protein
conservation.
The HTF significantly increased the acetic acid and decreased the butyric acid
concentra-
tions. There were significant two-way (variety × HMF and variety × HTF) and
three-way
(variety × HMF × HTF) interactions for crude protein content, fiber contents,
volatile fatty
acids profile, and digestibility characteristics (p < 0.05). For Karma 2000,
the application
of HMF increased crude protein concentration; however, it also increased lignin
and fiber contents, thereby reducing organic matter digestibility. In
contrast, dual inoculation raised
neutral detergent fiber digestibility. The results show that variety-specific
inoculant selec-
tion can improve triticale silage quality, underscoring the importance of
aligning microbial
additives with the forage’s biochemical properties.