Akademik Veri Yönetim Sistemi
14th International Symposium on Pharmaceutical Sciences (ISOPS), Ankara, Türkiye, 25 - 28 Haziran 2024, ss.2
GREEN SYNTHESIS OF SILVER AND ZINC NANOPARTICLES USING PROPOLIS EXTRACTS OBTAINED FROM GİRESUN REGION AND THEIR ANTIMICROBIAL, ANTIBIOFILM AND ANTI-QUARUM SENSING ACTIVITIES
1,2Vural N. 1,2Gökdere N., 3Rızvanoğlu SS., 4Doğanç F., 3,5Eryılmaz M.,1Palabıyık İM.
1Ankara University, Department of Analytical Chemistry, Ankara, Türkiye, nnazvural@gmail.com
2Ankara University,Graduate School of Health Sciences,Ankara, Türkiye ,nutan.gokdere@gmail.com
3Ankara University, Department of Pharmaceutical Microbiology, Ankara, Türkiye, ssgurpinar@ankara.edu.tr
4Ankara University, Department of Pharmaceutical Chemistry, Ankara, Türkiye, fdoganc@ankara.edu.tr
5Acibadem Mehmet Ali Aydinlar University, Department of Pharmaceutical Microbiology, İstanbul,Türkiye, Mujde.Eryilmaz@acibadem.edu.tr
Introduction: Propolis is a complex natural bee product containing phenolic compounds and flavonoids, obtained from plants by Apis mellifera L. (honey bee) (1).The green approaches are safe and environmentally friendly methods for nanoparticle synthesis.Within the scope of this study, AgNPs and ZnNPs were synthesized and characterized using a green method from the ethanol extract of propolis in Giresun region. Following these processes, their antimicrobial, antibiofilm and anti-quarum activities were examined.
Materials and Methods: Propolis extract was prepared in 70% ethanol. An appropriate amount of extract is added to the salt solutions. It is incubated and centrifuged(2).For the antimicrobial activity, the MIC values of the nanoparticles were determined using the broth microdilution method. The anti-QS activity test was performed by the disc diffusion method using reporter bacteria Chromobacterium violaceum ATCC 12472.The antibiofilm activity against P. aeruginosa PAO1 was evaluated using the crystal violet assay.
Results: Characterization study was carried out using UV-Vis and AgNPs and ZnNPs were detected at 420 and 320 nm, respectively.The SEM study showed that the NPs were below 100 nm. According to the antibacterial activity results, ZnNP and AgNPs showed MIC values of 0.0625 mg/ml and 0.0250 mg/ml against S. aureus ATCC 43300, respectively.The percentage biofilm inhibition value of ZnNPs and AgNPs was found to be 38.94% and 38.67%, respectively.
Conclusions: According to the characterization results, nanoparticles smaller than 100 nm were synthesized and no impurities were observed. The antimicrobial activity results, NPs showed the best activity against gram-positive bacteria.
References: 1. Bankova VS, De Castro SL, Marcucci MC, (2000). Apidologie, 31, 3 –15.
2. Barsola B, Kumari P, (2022). Green Processing and Synthesis, 11:659-673.