What happens to basophils and tryptase, LXA<sub>4</sub> and CysLTs during aspirin desensitization?

ÇELİK G. E., AYDIN Ö., Güloğlu D., Seçil D., MELLİ M., DOĞU E. F., ...Daha Fazla

JOURNAL OF ASTHMA, cilt.60, sa.8, ss.1524-1534, 2023 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 60 Sayı: 8
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1080/02770903.2022.2156352
  • Dergi Adı: JOURNAL OF ASTHMA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, Psycinfo, Veterinary Science Database
  • Sayfa Sayıları: ss.1524-1534
  • Anahtar Kelimeler: Aspirin desensitization, basophils, leukotrienes, CD203c, NERD, EXACERBATED RESPIRATORY-DISEASE, NONSTEROIDAL ANTIINFLAMMATORY DRUGS, ASTHMATIC-PATIENTS, CYSTEINYL-LEUKOTRIENES, INDUCED SPUTUM, HYPERSENSITIVITY, RHINOSINUSITIS, CHALLENGE, SENSITIVITY, ACTIVATION
  • Ankara Üniversitesi Adresli: Evet

Özet

IntroductionAspirin desensitization (AD) is an effective treatment in patients with non-steroidal anti-inflammatory drugs (NSAID)-exacerbated respiratory disease (NERD) by providing inhibitory effect on symptoms and polyp recurrence. However, limited data is available on how AD works. We aimed to study comprehensively the mechanisms underlying AD by examining basophil activation (CD203c upregulation), mediator-releases of tryptase, CysLT, and LXA(4), and LTB4 receptor expression for the first 3 months of AD.MethodsThe study was conducted in patients with NERD who underwent AD (group 1: n = 23), patients with NERD who received no desensitization (group 2: n = 22), and healthy volunteers (group 3, n = 13). All participants provided blood samples for flow cytometry studies (CD203c and LTB4 receptor), and mediator releases (CysLT, LXA(4,) and tryptase) for the relevant time points determined.ResultsAll baseline parameters of CD203c and LTB4 receptor expressions, tryptase, CysLT, and LXA(4) releases were similar in each group (p > 0.05). In group 1, CD203c started to be upregulated at the time of reactions during AD, and continued to be high for 3 months when compared to controls. All other study parameters were comparable with baseline and at the other time points in each group (p > 0.05).ConclusionAlthough basophils are active during the first 3 months of AD, no releases of CysLT, tryptase or LXA(4) exist. Therefore, our results suggest that despite active basophils, inhibition of mediators can at least partly explain underlying the mechanism in the first three months of AD.