Development of an In Situ Hybridization Method for Detection of Akabane Virus
JOURNAL OF BIOLOGICAL METHODS, cilt.11, sa.1, ss.1-5, 2024 (Hakemli Dergi)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 11 Sayı: 1
- Basım Tarihi: 2024
- Doi Numarası: 10.14440/jbm.2024.413
- Dergi Adı: JOURNAL OF BIOLOGICAL METHODS
- Derginin Tarandığı İndeksler: BIOSIS, CAB Abstracts
- Sayfa Sayıları: ss.1-5
- Ankara Üniversitesi Adresli: Evet
Özet
Akabane virus (AKAV) is an arbovirus belonging to the family Bunyaviridae, genus Orthobunyavirus. AKAV consists of three-segment (L, M, and S RNA segments), negative single-stranded RNA. The aim of this study was to investigate an in situ hybridization method (ISH) in a Vero E6 cell line infected with Akabane virus. The 320 base pair amplicon was obtained by RT-PCR with a primer pair and labeled with digoxigenin. Akabane virus RNAs were seen as a granular pattern in the cytoplasm of infected cells. As a result, the expression of the particular Akabane virus gene area was successfully disclosed in the current investigation using the ISH method with a digoxigenin-labeled probe.