Advanced three-dimensional morphometric analysis of calvarial bones used in scaffold studies
Annals of Anatomy, cilt.267, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 267
- Basım Tarihi: 2026
- Doi Numarası: 10.1016/j.aanat.2026.152876
- Dergi Adı: Annals of Anatomy
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, EMBASE, MEDLINE, Zoological Record
- Anahtar Kelimeler: Animal model selection, Biomaterial applications, Cranial bone thickness, Morphometric modeling, Three-dimensional imaging techniques
- Ankara Üniversitesi Adresli: Evet
Özet
Background Calvarial bones, particularly the frontal and parietal bones, play a critical role in craniofacial research, however, comprehensive guidelines on their thickness and morphometry remain lacking. Methods This study conducted detailed morphometric analyses using 3D reconstructions of 16 specimens each from New Zealand rabbits, domestic pigs, and Wistar rats (8 females and 8 males per species), as well as 20 specimens (8 females, 12 males) from Romanov sheep. Micro-CT imaging was utilized for rats and rabbits, while conventional CT imaging was used for sheep and pigs. Three-dimensional models were manually generated in 3D Slicer to measure precise bone thickness. Results The result showed that average calvarial thickness varied by species: rat (parietal 0.77 ± 0.01 mm, frontal 0.95 ± 0.02 mm), rabbit (frontal 2.19 ± 0.05 mm, parietal 1.72 ± 0.02 mm), sheep (parietal 7.75 ± 0.13 mm, frontal 7.49 ± 0.13 mm), pig (parietal 48.70 ± 7.16 mm, frontal 36.33 ± 5.06 mm). Our findings indicates that the rat skull, especially the parietal bone, is well-suited for anatomical and morphometric studies due to its consistent thickness and symmetrical structure. Among its bones, the parietal bone stands out as ideal for creating bone defect models due to its symmetrical structure and consistent thickness. Conclusion These findings offer valuable guidance for selecting appropriate animal models and designing scaffold materials, enhancing the accuracy and effectiveness of bone defect research and regenerative strategies.