Spectrochromatographic determination of dorzolamide hydrochloride and timolol maleate in an ophthalmic solution using three-way analysis methods

DİNÇ E., Buker E.

TALANTA, cilt.191, ss.248-256, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 191
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.talanta.2018.08.082
  • Dergi Adı: TALANTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.248-256
  • Anahtar Kelimeler: Parallel factor analysis, Trilinear partial least squares, Dorzolamide hydrochloride, Timolol Maleate, Ophthalmic formulation, 1ST-DERIVATIVE UV-SPECTROPHOTOMETRY, PERFORMANCE LIQUID-CHROMATOGRAPHY, PRESSURE CHEMICAL-IONIZATION, DE-ETHYLATED METABOLITE, CARBONIC-ANHYDRASE, MASS-SPECTROMETRY, MULTILINEAR PLS, PLASMA, RESOLUTION, DATASET
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study, the simultaneous spectrochromatographic determination of dorzolamide hydrochloride (DOR) and timolol maleate (TMM) in an ophthalmic formulation was performed by applying multiway resolution methods, parallel factor analysis (PARAFAC) and three way partial least squares (3W-PIS) models. In similar manner, unfolded partial least squares (U-PLS) approach was applied to the UPLC analysis of the related drugs. For the three-way resolutions, the ultra-performance liquid chromatography-photodiode array (UPLC-PDA) data array of the calibration samples and others (validation and commercial samples) containing the analyzed drugs (DOR and TMM) and thiamine hydrochloride as an internal standard (IS) was decomposed into triads or trilinear components. These three different calibration models were employed for the spectrochromatographic resolution of a commercial ophthalmic formulation of DOR and TMM and then successful quantitation results were obtained. Analysis results provided by applying three-way and two-way resolutions were statistically compared to those obtained by the newly developed standard UPLC approach. It was observed that the proposed methods gave comparable determination results for analysis of the DOR-TMM ophthalmic formulation.