Research Information System
INTERNATIONAL SYMPOSIUM ON PHARMACEUTICAL SCIENCES, Ankara, Turkey, 25 - 28 June 2024, pp.86
Introduction: Quercetin is a phyto-active compound with remarkable pharmacological potential, however it has limited oral bioavailability. In order to increase its absorbtion through gastro-intestinal system many strategies have been examined by means of various drug delivery systems (1). Phytosomes can be given as an example for phospholipid-based carriers among these delivery systems. The aim of this study was to demonstrate the characterization of quercetin loaded phytosomes in in vitro conditions. Materials and Methods: Quercetin and L-α-Phosphatidylcholine (PC) from egg yolk were purchased from Sigma. Solvent evaporation/thin film formation method was used to prepare phytosomes (2). Quercetin phospholipid ratios, process temperature (40, 50, 60 oC) and reaction time (1,2,3 h) were used as formulation parameters. In order to characterize the formulations, encapsulation efficiency (EE%), particle size, polydispersity index and zeta potential were determined. In direct method was used for the determination of entrapment efficiency. Among all the prepared formulas, the three formulas with the highest loading efficiency were chosen (Table 1). X-ray Diffraction (XRD) of these formulations was determined Results: In the formulations as the PC ratios increased, particle size and PDI values decreased, but EE% increased. ZPs were independent. The absence of a Quercetin peak for XRD results indicates that there was an amorphous structure of the active ingredient in the formulation and phytosome structure formed. The results of the formulations were shown in Table 1.