Investigation of the anticancer effects of Lobaria pulmonaria on breast cancer cells, including antiproliferative effects, apoptosis induction, and cytoskeletal damage Investigación de los efectos anticancerígenos de Lobaria pulmonaria en células de cáncer de mama, incluyendo efectos antiproliferativos, inducción de apoptosis y daño citosquelético

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Yumrutas O., Guven C., Yöntem F. D., Ozay Y., Şener L. T., Ahbab M. A., ...Daha Fazla

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas, cilt.24, sa.6, ss.1015-1022, 2025 (SCI-Expanded, Scopus)

• Yayın Türü: Makale / Tam Makale
• Cilt numarası: 24 Sayı: 6
• Basım Tarihi: 2025
• Doi Numarası: 10.37360/blacpma.25.24.6.68
• Dergi Adı: Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas
• Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Fuente Academica Plus, BIOSIS, EMBASE
• Sayfa Sayıları: ss.1015-1022
• Anahtar Kelimeler: Apoptosis, Breast cancer, F-actin, Lichen, Lobaria pulmonaria
• Ankara Üniversitesi Adresli: Evet

Özet

Breast cancer is the leading cause of death among women. Natural compounds play an important role in the prevention of breast cancer. In this study, the anticancer properties of Lobaria pulmonaria were investigated. Cell viability and proliferation were assessed using MTT test, apoptotic cell percentages were determined by AnnexinV/PI assay, protein levels of BCL2, CASP3 and P53 were measured by western blot. Additionally, F-actin levels were analyzed using Immunofluorescence staining. It was determined that L. pulmonaria reduced the viability of MCF7 cells. While the percentage of apoptotic cells did not change, the percentage of non-apoptotic cell death increased. Moreover, there were no significant changes in BCL2, CASP3, and P53 protein levels, nor in the amount of F-actin. In conclusion, L. pulmonaria did not induce apoptosis in MCF7 breast cancer cells but appeared to trigger a non-apoptotic cell death pathway, such as necrosis.