Akademik Veri Yönetim Sistemi
ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, sa.3, ss.197-202, 2012 (SCI-Expanded)
Porcine Reproductive and Respiratory Syndrome (PRRS) is an important disease that causes severe economic losses in pig industry. PRRSV has two genotypes named as a European (EU) and North American (US). PRRSV appears globally a variety of countries including Canada, USA, South Korea, Germany, Spain, UK, Denmark and Greece. Furthermore, US genotype has been detected serologically in France, Germany and USA in wild boars. So far, no comprehensive information has been generated in Turkey in terms of presence and/or epidemiology of the disease. The aim of this study was to determine presence of PRRSV at initial step and subsequently provide information on possible genotypes that might have occurred in domestic pig and wild boars. The study was carried out on 86 nasal swaps from two different farms and 12 lungs tissue samples from wild boars. A total of 71.4% of samples were found PRRSV- positive by one-step RT-PCR. Prevalence of the virus in Farms 1 and 2 and wild boars was determined as 76.9%, 61.9% and 58%, respectively. Nucleotide sequence analyses performed on ORF 7 of the genome showed that 96.5% and 98.2%, nucleotide homologies in Izmir and Mersin, respectively. In addition, phylogenetic analysis showed that all Turkish PPRSVs was located in the lineage of US like PRRS viruses. This is the first report of PRRSV infection between domestic pigs and wild boars in Turkey.