Atıf İçin Kopyala
Cheraghi S., Taher M. A., Karimi-Maleh H., Karimi F., Shabani-Nooshabadi M., Alizadeh M., ...Daha Fazla
CHEMOSPHERE, cilt.287, 2022 (SCI-Expanded)
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Yayın Türü:
Makale / Tam Makale
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Cilt numarası:
287
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Basım Tarihi:
2022
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Doi Numarası:
10.1016/j.chemosphere.2021.132187
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Dergi Adı:
CHEMOSPHERE
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Derginin Tarandığı İndeksler:
Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), Artic & Antarctic Regions, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Environment Index, Food Science & Technology Abstracts, Geobase, Greenfile, MEDLINE, Metadex, Pollution Abstracts, Public Affairs Index, Veterinary Science Database, Civil Engineering Abstracts
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Anahtar Kelimeler:
Glutathione peroxidase, Enzymatic biosensor, Glutathione, Graphene oxide, Nafion, Voltammetric method, ELECTROCHEMICAL DETECTION, URIC-ACID, ELECTRODE, NANOPARTICLES, IMMOBILIZATION, NANOCOMPOSITE, SENSORS, GOLD
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Ankara Üniversitesi Adresli:
Evet
Özet
In this work, we report a novel enzymatic biosensor based on glutathione peroxidase (GSH-Px), graphene oxide (GO) and nafion for the electrochemical sensing of glutathione (GSH) in body fluids. GSH-Px was immobilized covalently via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) onto modified glassy carbon electrode (GCE) decorated with GO and nafion and successfully used for sensing of GSH in the presence of H2O2 as catalyst with Michaelis-Menten constant about 0.131 mmol/L. The active surface are of GCE improve from 0.183 cm2 to 0.225 cm2 after modification with GO. The introduced biosensor (GSH-Px/ GO/nafion/GCE) was used for monitoring of GSH over the range 0.003-370.0 mu M, with a detection limit of 1.5 nM using differential pulse voltammetric (DPV) method. The GSH-Px/GO/nafion/GCE was successfully applied to the determination of GSH in real samples.