Voltammetric behaviour and determination of moxifloxacin in pharmaceutical products and human plasma
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, vol.378, no.5, pp.1351-1356, 2004 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 378 Issue: 5
- Publication Date: 2004
- Doi Number: 10.1007/s00216-003-2427-7
- Journal Name: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.1351-1356
- Keywords: moxifloxacin, cyclic voltammetry, differential pulse voltammetry, Osteryoung square-wave voltammetry, high-performance liquid chromatography, human plasma, IN-VITRO ACTIVITY, PERFORMANCE LIQUID-CHROMATOGRAPHY, BAY 12-8039, HUMAN URINE, 8-METHOXYQUINOLONE, TROVAFLOXACIN, LEVOFLOXACIN
- Ankara University Affiliated: Yes
Abstract
The oxidative behaviour of moxifloxacin was studied at a glassy carbon electrode in different buffer systems using cyclic, differential pulse, and Osteryoung square-wave voltammetry. The oxidation process was shown to be irreversible over the entire pH range studied (2.0-10.0) and was diffusion-controlled. The methods were performed in Britton-Robinson buffer and the corresponding calibration graphs were constructed and statistical data were evaluated. When the proposed methods were applied at pH 6.0 linearity was achieved from 4.4x10(-7) to 1.0x10(-5) mol L-1. Applicability to tablets and human plasma analysis was illustrated. Furthermore, a high-performance liquid chromatographic method with diode-array detection was developed. A calibration graph was established from 4.0x10(-6) to 5.0x10(-5) mol L-1 moxifloxacin. The described methods were successfully employed with high precision and accuracy for estimation of the total drug content of human plasma and for pharmaceutical dosage forms of moxifloxacin.