Transcriptional Regulation of stj Fimbrial Operon in Salmonella Typhimurium

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Yuksel D., AKÇELİK N., AKÇELİK M.

ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.59, sa.1, ss.65-70, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 59 Sayı: 1
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1501/vetfak_0000002503
  • Dergi Adı: ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.65-70
  • Anahtar Kelimeler: Typhimurium, stj operon, cadC, transcriptional activator, COLI-CAD OPERON, ACID TOLERANCE, EXPRESSION, IDENTIFICATION, CADAVERINE, SEQUENCE, PROTEIN
  • Ankara Üniversitesi Adresli: Evet

Özet

The stj fimbrial operon harbors five genes stjABCDE. The operon plays role in the persistence and virulence of S. Typhimurium in host systems. In this study, in vitro activation of the stj operon was achieved by transposon (TPOP) mutagenesis. The transposon insertion site was located within the cadC gene promoter in the stj operon-activated mutant strain MDN20. Comparative analysis of two-dimensional gel electrophoresis protein patterns of the wild type strain (S. Typhimurium LT2) and mutant strain MDN20 revealed that the CadC and StjB proteins were produced by mutant strain MDN20 but not by the wild type strain. These results were also confirmed by Maldi-TOF analysis. These findings indicate that cadC gene is a transcriptional activator of stj fimbrial operon in S. Typhimurium.