Exploring allosteric coupling in the alpha-subunit of Heterotrimeric G proteins using evolutionary and ensemble-based approaches

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SAYAR K., Ugur Ö., Liu T., Hilser V. J., ONARAN H. O.

BMC STRUCTURAL BIOLOGY, cilt.8, 2008 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 8
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1186/1472-6807-8-23
  • Dergi Adı: BMC STRUCTURAL BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Ankara Üniversitesi Adresli: Evet

Özet

Background: Allosteric coupling, which can be defined as propagation of a perturbation at one region of the protein molecule (such as ligand binding) to distant sites in the same molecule, constitutes the most general mechanism of regulation of protein function. However, unlike molecular details of ligand binding, structural elements involved in allosteric effects are difficult to diagnose. Here, we identified allosteric linkages in the alpha-subunits of heterotrimeric G proteins, which were evolved to transmit membrane receptor signals by allosteric mechanisms, by using two different approaches that utilize fundamentally different and independent information.