Development and validation of an RP-HPLC method for the determination of valacyclovir in tablets and human serum and its application to drug dissolution studies

Savaser A., Ozkan C., Ozkan Y., Uslu B., Ozkan S. A.

Journal of Liquid Chromatography and Related Technologies, cilt.26, sa.11, ss.1755-1767, 2003 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Özet
  • Cilt numarası: 26 Sayı: 11
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1081/jlc-120021283
  • Dergi Adı: Journal of Liquid Chromatography and Related Technologies
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1755-1767
  • Anahtar Kelimeler: valacyclovir, HPLC, determination, pharmaceuticals, human serum, dissolution profile, VALACICLOVIR, ACYCLOVIR
  • Ankara Üniversitesi Adresli: Evet

Özet

A specific, sensitive, simple, and rapid HPLC method has been developed for the determination of valacyclovir (VACL) in raw material, pharmaceutical dosage forms, and human serum, in order to carry out drug dissolution studies from tablets. The chromatographic separation was achieved with acetonitrile: methanol: 0.067 M KH2PO4 (27:20:53, v/v/v) adjusted to pH 6.5 with 3 M NaOH as mobile phase, a Waters Spherisorb C18 column, and UV detection at 244 nm. Etodolac was used as an internal standard. Linearity range was 5-20,000ngmL-1. Limit of detection obtained was 0.38 and 0.14ngmL-1 in mobile phase and spiked human serum samples, respectively. The described method can be readily applied, without any interferences from the excipients, for the determination of the drug in tablets, human serum samples, and drug dissolution studies.