Akademik Veri Yönetim Sistemi
Turkiye Klinikleri Journal of Medical Sciences, cilt.33, sa.5, ss.1308-1315, 2013 (SCI-Expanded)
Objective: This study aims to compare ELISA (Enzyme-Linked Immonosorbent Assay) and DFA (Direct Florescent Antibody) methods with direct microscopy, and to evaluate the place of ELISA and DFA in routine diagnosis of Giardia intestinalis. Material and Methods: The study population consisted of 150 patients who applied to Gaziantep University Şahinbey Research Hospital polyclinics complaining for acute, chronic or recurrent diarrhea, and their stool specimens were first analyzed with direct microscopy (native-lugol), and then with ELISA and DFA. Results: In 22 out of 150 stool specimens (14.7%), G. intestinalis cyst/trophozoites were seen on direct microscopy, and this was confirmed positive with ELISA and DFA. In 6 out of 128 specimens (4.6%) analyzed with ELISA and in 4 out of 128 (3.1%) analyzed with DFA cryst/trophozoites were diagnosed as positive. When compared to direct microscopy, ELISA and DFA's sensitivity, specificity, negative/positive predictive values and consistency can be sequenced in terms of kappa values (κ) as: 78.57%, 100%, 100%, 95.31%, 0.821, and 84.62%, 100%, 100%, 96.87%, 0.86 (p<0.001); when ELISA is compared to DFA, DFA?s values can be sequenced respectively as: 100%, 98.38%, 92.85%, 100% and 0.915 (p<0.001). Conclusion: It can be concluded that the use of direct microscopic method in the diagnosis of G. intestinalis should be preferred at the beginning. ELISA should be done when extensive epidemiological research is performed, and symptomatic parasites cannot be detected; and DFA should be applied when verification is needed for the diagnosis of giardiasis. © 2013 by Türkiye Klinikleri.