Separation kinetics of L-phenylalanine by ion-exchange process

Takac S., Calik G., Aytar M., Ozdamar T.

BIOCHEMICAL ENGINEERING JOURNAL, cilt.2, sa.2, ss.101-112, 1998 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 2 Sayı: 2
  • Basım Tarihi: 1998
  • Doi Numarası: 10.1016/s1369-703x(98)00022-9
  • Dergi Adı: BIOCHEMICAL ENGINEERING JOURNAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.101-112
  • Anahtar Kelimeler: ion-exchange equilibrium, ion-exchange kinetics, L-phenylalanine separation, TRANS-CINNAMIC ACID, AMINO-ACIDS, RHODOTORULA-GLUTINIS, VARYING DEGREE, CATION RESINS, CROSS-LINKING, AMMONIA-LYASE, EQUILIBRIUM, DIPEPTIDES
  • Ankara Üniversitesi Adresli: Evet

Özet

Ion-exchange kinetics of L-phenylalanine (Phe) by strongly acidic cation-exchange resin Amberlite IR 120-P was investigated. Phe(+)/H+ equilibrium isotherms were obtained in a wide range of pH and temperature and the related equilibrium models were determined. The effects of pH, temperature, feed stream volumetric flow rate and feed stream Phe concentration were investigated in a differential ion-exchange column (DIC) and pH=5.0, 20 degrees C temperature, 1.0x10(-2) dm(3) min(-1) feed rate and 45 mmol dm(-3) feed Phe concentration were found to be the favourable values. Rate limiting step of the ion-exchange process varied depending on the operation conditions and the overall internal-external mass transfer coefficients were calculated. Phe(+)/H+ ion-exchange was also performed in an integral ion-exchange column at different feed rates, column lengths and flow directions and favourable breakthrough curves were obtained. The results were used for the separation of Phe from the bioreaction medium, which consists of trans-cinnamic acid and ammonia, and the separation was successfully achieved in DIG. (C) 1998 Elsevier Science S.A. All rights reserved.