RAPD analysis of genetic relations between Buzgulu grape cultivars (Vitis vinifera) grown in different parts of Turkey

Aras S., Polat J., Cansaran D., SÖYLEMEZOĞLU G.

ACTA BIOLOGICA CRACOVIENSIA SERIES BOTANICA, cilt.47, sa.2, ss.77-82, 2005 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 47 Sayı: 2
  • Basım Tarihi: 2005
  • Dergi Adı: ACTA BIOLOGICA CRACOVIENSIA SERIES BOTANICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.77-82
  • Anahtar Kelimeler: Vitis vinifera L., Buzgulu, polymorphism, RAPD, Turkey, IDENTIFICATION, DISCRIMINATION, CLONES
  • Ankara Üniversitesi Adresli: Evet

Özet

The genetic relations between Buzgulu grape ecotypes (Vitis vinifera L.) grown in different regions of Turkey were investigated by RAPD analysis. Of 50 decamer primers tested, 20 were used in the study, and 17 yielded clear and reproducible polymorphic bands. Differences between ecotypes were analyzed statistically. The similarity index was lowest (0.601) between Etli Buzgulu (collection no. 619, from Antalya province) and Buzgulu (no. 572, Eskisehir prov.), and highest (0.879) between Sik Buzgulu (no. 516, Konya prov.) and Buzgulu (no. 738, Kytahya prov.). The data matrix indicated genetic distances ranging up to 0.038, although most were under 0.03. Phylogenetic trees obtained by UPGMA and neighbor-joining methods basically resembled each other, with some differences. The results from RAPD analysis correlated with some morphological characteristics. This study of Turkish grape germplasm suggests that the geographical and ecological distribution of the plants contributes to higher genetic similarity.