In vitro micropropagation from plumular apices of Turkish cowpea (Vigna unguiculata L.) cultivar Akkiz

Aasim M., Khawar K. M., ÖZCAN S.

SCIENTIA HORTICULTURAE, cilt.122, sa.3, ss.468-471, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 122 Sayı: 3
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1016/j.scienta.2009.05.023
  • Dergi Adı: SCIENTIA HORTICULTURAE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.468-471
  • Anahtar Kelimeler: Cowpea, Micropropagation, Plumular apices, Pulse treatment, CELL-SUSPENSION CULTURES, GENETIC-TRANSFORMATION, AGROBACTERIUM-TUMEFACIENS, SOMATIC EMBRYOGENESIS, PLANT-REGENERATION, COTYLEDON EXPLANTS, GRAIN LEGUMES, L WALP, TRANSMISSION, TRANSGENES
  • Ankara Üniversitesi Adresli: Evet

Özet

Multiple shoot formation from the plumular apices excised from mature embryos of cowpea cv. Akkiz was obtained after pulse treatment with 10 mg/l BAP for 5 days followed by culture on MS medium containing 0.25, 0.50, 0.75 and 1.00, 1.25 mg/l BAP - with or without 0.10 mg/l NAA. Callus induction and shoot regeneration was recorded on all cultures containing BAP with or without NAA. However, inclusion of 0.1 mg/l NAA had positive effect on callus diameter and shoot length. Maximum mean number of 7.11 shoots per explant were obtained on MS medium containing 1.00 mg/l BAP. Longer shoots were recorded on MS medium containing various concentration of BAP + 0.1 mg/l NAA compared to those containing various concentrations of BAP singly. All shoots cultured on MS medium containing I mg/l BAP were rooted on MS medium containing 0.50 mg/l IBA. Rooted plants were acclimatized at room temprature in soil contained in pots. All plants flowered and set seeds in the greenhouse after 3 months. (C) 2009 Elsevier B.V. All rights reserved.