Classification of virulent lactococcal bacteriophages based on protein composition and restriction endonuclease analysis

Sanlibaba P., Akcelik M.

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, cilt.29, sa.3, ss.865-871, 2005 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 3
  • Basım Tarihi: 2005
  • Dergi Adı: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.865-871
  • Anahtar Kelimeler: Lactococcus lactis, bacteriophage, protein profile, restriction analysis, LACTIC-ACID BACTERIA, PHAGE RESISTANCE, TAXONOMIC DIFFERENTIATION, IDENTIFICATION, STREPTOCOCCI
  • Ankara Üniversitesi Adresli: Evet

Özet

Twenty-four virulent bacteriophages isolated from raw milk and whey samples were characterised by structural protein composition and bacteriophage DNA restriction endonuclease fragments. Twenty-four lactococcal bacteriophages were possessed among 24-31 total structural proteins with molecular masses of 6.5-208.9 kDa and 2-8 major structural proteins with molecular masses of 6.5-38 kDa. Lactococcal bacteriophages were classified as 5 different groups by total structural proteins and 4 different groups by major structural proteins. The genome sizes of bacteriophages were estimated between 19.6 and 39 kb. Twenty-four lactococcal bacteriophages were classified into 8 groups based on the same restriction endonuclease recognition and restriction sites on the lactococcal bacteriophage genomes.