A novel amperometric biosensor based on ZnO nanoparticles-modified carbon paste electrode for determination of glucose in human serum

AYDOĞDU TIĞ G., KOYUNCU ZEYBEK D., Pekyardimci S., Kilic E.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, cilt.41, sa.5, ss.332-338, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41 Sayı: 5
  • Basım Tarihi: 2013
  • Doi Numarası: 10.3109/21691401.2012.744994
  • Dergi Adı: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.332-338
  • Anahtar Kelimeler: amperometric biosensor, carbon paste electrode, glucose, human serum, ZnO nanoparticles, URIC-ACID, ELECTROCHEMICAL BIOSENSORS, COMPOSITE ELECTRODE, OXIDASE, IMMOBILIZATION, NANOTUBES, SENSOR, CHITOSAN, FILM, ELECTROCATALYSIS
  • Ankara Üniversitesi Adresli: Evet

Özet

Zinc oxide nanoparticles-(ZnONPs) modified carbon paste enzyme electrodes (ZnONPsMCPE) were developed for determination of glucose. The determination of glucose was carried out by oxidation of H2O2 at +0.4 V. ZnONPsMCPE provided biocompatible microenvironment for GOx and necessary pathway of electron transfer between GOx and electrode. The response of GOx/ZnONPsMCPE was proportional to glucose concentration and detection limit was 9.1 X 10(-3) mM. K-m and I-max, were calculated as 0.124 mM and 2.033 mu A. The developed biosensor exhibits high analytical performance with wide linear range (9.1. X 10(-3) -14.5 mM), selectivity and reproducibility. Serum glucose results allow us to ascertain practical utility of GOx/ZnONPsMCPE biosensor.