Application of cellulose 3,5-dichlorophenylcarbamate covalently immobilized on superficially porous silica for the separation of enantiomers in high-performance liquid chromatography

Bezhitashvili L., Bardavelidze A., Mskhiladze A., GÜMÜŞTAŞ M., ÖZKAN S. A., Volonterio A., ...Daha Fazla

JOURNAL OF CHROMATOGRAPHY A, cilt.1571, ss.132-139, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1571
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1016/j.chroma.2018.08.011
  • Dergi Adı: JOURNAL OF CHROMATOGRAPHY A
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.132-139
  • Anahtar Kelimeler: Enantioseparations, Chiral HPLC, Chiral stationary phase based on superficially porous silica, Covalently immobilized polysaccharide-based chiral stationary phase, CHIRAL STATIONARY PHASES, CORE-SHELL, CAPILLARY COLUMNS, HPLC SEPARATION, HIGH-EFFICIENCY, ENANTIOSEPARATIONS, PARTICLES, TRIS(3,5-DICHLOROPHENYLCARBAMATE), DERIVATIVES, AMYLOSE
  • Ankara Üniversitesi Adresli: Evet

Özet

Our earlier studies have demonstrated the applicability of polysaccharide-based chiral selectors in combination with superficially porous (or core-shell) silica (SPS) particles for the preparation of highly efficient chiral stationary phases (CSP). In earlier studies, CSPs were prepared by coating (adsorption) of the chiral selector onto the surface of silica. In this study we report for the first time the CSP obtained by covalent immobilization of a chiral selector onto the surface of SPS particles. The applicability of this CSP for the separation of enantiomers in pure methanol and acetonitrile, as well as in n-hexane/2-propanol mobile phases is shown. The effect of the injected sample amount, mobile phase flow rate and detection frequency on separation performance were studied, as well as high efficiency separation of enantiomers with the analysis time less than 30 s was attempted. (C) 2018 Elsevier B.V. All rights reserved.