Akademik Veri Yönetim Sistemi
European Academy of Forensic Science 2025 (EAFS 2025), Dublin, İrlanda, 26 - 30 Mayıs 2025, ss.20, (Özet Bildiri)
The time elapsed after death is known as the postmortem interval (PMI). After death, the body undergoes changes such as algor mortis,
rigor mortis, livor mortis, and putrefaction. In forensic medicine, PMI is traditionally determined using standard methods and forensic entomology, both of
which can be affected by environmental conditions. Recently, genetic materials, which are less influenced by the environment, have gained attention in PMI
studies. Cell-free DNA (cfDNA) comprises short DNA fragments released into the bloodstream due to cell necrosis and apoptosis. This study quantitatively
analyzed cfDNA and specific microRNAs (miRNAs) — miR-122, miR-133, miR-1, miR-16, and let-7e — in serum samples from rats at various time intervals to
assess their correlation with PMI. The results were analyzed using Ct, ΔCt, ΔΔCt, and fold change methods. Statistically, miR-16 and miR-122 had lower ΔCt
values at all time points. miR-16 showed a lower ΔΔCt average at 24 and 48 hours and the highest fold change at 24 hours. Reference genes GAPDH and
ACTB declined over time, with higher averages at 0, 6, and 12 hours compared to 24 and 48 hours. This research is the first to quantitatively explore
cfDNA’s potential role in PMI estimation.