Erkoç E., Taliboğlu S., Korkmaz F., Eskizengin H., Kıran F., Koçberber Kılıç N.
JOURNAL OF APPLIED BIOLOGICAL SCIENCES, cilt.20, sa.2, ss.55-68, 2026 (Hakemli Dergi)
Özet
Biogenic AgNP production at room temperature (20 ± 2 °C) and 37 °C were investigated using Stenotrophomonas maltophilia. Transmission electron microscopy showed average particle sizes of 9.1 ± 2.6 nm at room temperature and 6.8 ± 1.8 nm at 37 °C. ATR-FTIR analysis indicated that the nanoparticles were associated with biomolecules, mainly proteins and lipids, suggesting a role in stabilization. The synthesized AgNPs exhibited antibacterial activity against Staphylococcus aureus ATCC 29213 (17 ± 0.2 mm inhibition zone) and 84% antibiofilm activity against Pseudomonas aeruginosa. Photocatalytic results showed 57% removal of methylene blue within 20 minutes. Cytotoxicity assays revealed a dose-dependent response, with the strongest effect in A549 cells (IC₅₀: 6.54 µg/mL). All experiments were performed in at least biological triplicates, and statistical significance was evaluated where applicable (p < 0.05). These findings highlight the multifunctional nature of biogenic AgNPs and support their relevance for antimicrobial and environmental applications within the scope of in vitro conditions.