Analysis of the relationship between grapevine cultivars, sports and clones via DNA fingerprinting

Ye G., SÖYLEMEZOĞLU G., Weeden N., Lamboy W., Pool R., Reisch B.

VITIS, cilt.37, sa.1, ss.33-38, 1998 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 37 Sayı: 1
  • Basım Tarihi: 1998
  • Dergi Adı: VITIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.33-38
  • Anahtar Kelimeler: RAPD, PCR, polymorphism, cultivar identification, Vitis, wine grapes, juice grapes, FRAGMENT-LENGTH-POLYMORPHISMS, VITIS-VINIFERA, RAPD MARKERS, MICROSATELLITE REPEATS, ARBITRARY PRIMERS, GENETIC-ANALYSIS, RFLP ANALYSIS, IDENTIFICATION, INHERITANCE
  • Ankara Üniversitesi Adresli: Evet

Özet

DNA fingerprinting utilizing RAPD polymorphisms was employed to investigate the relationship among 16 grapevine cultivars and sports thought to have arisen from these cultivars. From 53 primers, a total of 464 bands were generated, of which 29 % were common to all genotypes tested. Cluster analysis classified all tested cultivars into two main groups (Vitis vinifera L. and V. x Labruscana Bailey) as expected. No polymorphism was detected among known clones of Chardonnay (Ch. clone 7, Ch. clone 78 and Ch. Geneva clone) or Pinot noir (P. n. clone 29, P. n. Geneva clone and P. n. Pernand). Pinot Meunier, Pinot gris, and Gamay Beaujolais displayed patterns indistinguishable from Pinot noir. Auxerrois and Melon showed unique patterns and may be classified as distinct cultivars. Chardonnay clone 7 shared 84 % of its bands with Pinot noir. There was more than 97 % RAPD amplicon homology between Niagara and two supposed sports, and between Concord and a red-fruited sport. Taking into account the error rate in scoring RAPD bands, the evidence is against the hypothesis that the three sports are distinct cultivars. While RAPD banding patterns could not distinguish between known clones, they were useful for distinguishing between phenotypically similar cultivars and for assessing the origins of cultivars thought to have originated as sports.