Mating type groups of Ascochyta rabiei (Teleomorph : didymella rabiei), the causal agent of chickpea blight in central Anatolia

Bayraktar H., Dolar F. S., Maiden S.

TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, cilt.31, sa.1, ss.41-46, 2007 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 1
  • Basım Tarihi: 2007
  • Doi Numarası: 10.3906/tar-0605-7
  • Dergi Adı: TURKISH JOURNAL OF AGRICULTURE AND FORESTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.41-46
  • Anahtar Kelimeler: chickpea, ascochyta rabiei, multiplex PCR, mating type, RESISTANCE, DEBRIS, STRAW, LOCUS, SPAIN
  • Ankara Üniversitesi Adresli: Evet

Özet

The distribution of mating types of Ascochyta rabiei (teleomorph: Didymella rabies) was studied on isolates collected from chickpea growing areas of Central Anatolia. Mating type assessments of 45 isolates from 6 different provinces were conducted under laboratory conditions. After keeping for 5-6 weeks, the pseudothecial development and ascospore production were observed on chickpea stem pieces that were incubated in 8 +/- 1 degrees C and 85% relative humidity. Both mating types were found in all the provinces, except for Kayseri and Sivas. The majority of the isolates belonged to Mat 1-1 (57.8%) and the others (42.2%) to MAT 1-2. Additionally, the mating types of A. rabiei were differentiated from each other using the multiplex PCR method with the primers Com1, Tail 5, and SP21. MAT 1-1 isolates were amplified to approximately a 460 by fragment and MAT 1-2 isolates to a 700 by fragment, in accordance with the discrimination of mating type groups with conventional methods.