Preparation of molecularly imprinted bilayer cryogel columns for selective protein depletion

Zenger O., Pesint G.

PROCESS BIOCHEMISTRY, cilt.117, ss.90-100, 2022 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 117
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.procbio.2022.03.018
  • Dergi Adı: PROCESS BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Compendex, Food Science & Technology Abstracts, INSPEC, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.90-100
  • Ankara Üniversitesi Adresli: Hayır

Özet

In this study, bilayer-imprinted cryogels were synthesized for depletion of human serum albumin (HSA) from human serum. For this purpose, a monolayer HSA-imprinted cryogel with 8% monomer concentration was synthesized and second imprinting process was performed using a polymeric solution with 16% monomer concentration in presence of HSA, in the supermacroporous structure of first layer. Thereby, bilayer HSAimprinted cryogel column (HSA-BLIP) with enhanced surface area was obtained. Bilayer non-imprinted polymer (BL-NIP) and monolayer HSA-imprinted polymers with monomer concentrations of 8% and 16% (HSAMIP(8%) and HSA-MIP16%, respectively) were synthesized as the control group. Synthesized columns were characterized by swelling tests, scanning electron microscopy (SEM), multipoint Brunauer-Emmett-Teller (BET) surface area measurement and micro computed tomography (Micro-CT) analysis. Surface areas of HSA-BLIP, BL NIP, HSA-MIP8% and HSA-MIP(16% )were found to be 39 m(2)/g, 37 m(2)/g, 21 m(2)/g and 12 m(2)/g, respectively. Maximum HSA adsorption was calculated as 106.8 mg/g for HSA-BLIP and 5.14 mg/g for BL-NIP. Selectivity studies were conducted in presence of competitor molecules, human hemoglobin (Hb) and immunoglobulin G (IgG). Finally, selective depletion of HSA directly from human serum was studied. In addition, it was demonstrated that HSA-BLIPs can be used up to 10 times without significant decrease in selective adsorption capacity.