Akademik Veri Yönetim Sistemi
European Wound Mangagement Association 2024, London, İngiltere, 1 - 05 Mart 2024, ss.1
Aim:This study aims to fabricate a postbiotic-loaded/human decellularized skin extracellular matrix (HdsECM)/alginate sprayable hydrogel to inhibit bacterial infections and enhance wound healing.
Method: Staphylococcus hominis EIR/HS-1 isolated from healthy human skin microbiota and identified by molecular techniques, was used as a source of postbiotics. Following the fermentation process, postbiotics were obtained by centrifugation and then lyophilization (A). The anti-microbial activity was tested against Methisillin-resistant Staphylococcus aureus-MRSA ATCC 43300, Pseudomonas aeruginosa 27853, and Staphylococcus epidermidis ATCC 35984 by agar well diffusion method and minimum inhibitory concentration (MIC) was determined. The antimicrobial effect of postbiotics against the skin pathogens was also carried out using florescent Live/Dead staining after 2, 6, 12, and 24 h treatments.
Results / Discussion: Postbiotics obtained from S. hominis EIR/HS-1 exhibited strong antibacterial activity against skin pathogens, tested. According to the results of the live-dead staining assay during co-incubation of postbiotics with each pathogen, it was shown under fluorescent microscope that live cells (green) were decreased time-dependently, and cell viability was all inhibited after 24h. To determine the biocompatibility of the hydrogel, HaCaT cells were cultured on it and then the cell viability was analyzed under the fluorescent microscope. Our results showed that postbiotics increased the cell viability on hydrogel compared to control groups. The results of tensile and compression analysis.
Conclusion: In conclusion, hybrid skin hydrogels containing decellularized human skin extracellular matrix and postbiotics from human skin microbiota should be used in skin regenerative and reconstructive medicine applications within the scope of wound management with their multi-functional roles.