Akademik Veri Yönetim Sistemi
Turk Hijyen ve Deneysel Biyoloji Dergisi, cilt.74, sa.2, ss.113-120, 2017 (Scopus)
Objective: Various viral vectors have been developed in order to delivery genes to living cells. Sendai virus (SeV) vectors are important viral vectors due to their properties suitable for gene delivery including transient gene expression, wide host cell specificity, low pathogenicity and strong immunogenicity. SeVs vectorss are highly used in molecular medicine in gene therapy, vaccine technology and regenerative. Methods: It was evaluated the gene delivery efficiency of SeV particles in various melanoma cell lines by using fluorescence microscope and confocal laser scanning microscope imaging techniques. A375, MDA-MB-435, G361 and WM115 cells have been transduced with SeV vectors expressing green fluorescent protein (GFP) at different multiplicity of infections (MOI): 1, 3, and 9. GFP expression was checked at 24 and 48 hours later following transduction. Confocal laser scanning microscopy imaging was calculated to gene delivery efficiency. Results: It was showed that A375, MDA-MB-435, G361 and WM115 cells are efficiently tranduced by seV even at low virus concentration with fluorescence microscopy imaging. GFP reporter gene activity started to be observed in 24 hours and peaked in 48 hours following viral transduction. Slight toxicity was observed following viral transduction in all cell 24 hours later; however, cells recovered and proliferated resulting in efficient gene expression 48 hours later. According to the confocal laser scanning microscopy imaging, more than 80% of all cell lines expressed GFP 48 hours after viral transduction. Conclusion: In conclusion, SeV vectors successfully transduced and expressed GFP reporter gene in various melanoma cell lines with high efficiency. This study discovered the use of SeV vectors in melanomaoriginated cells and it can open up wide range of studies involving SeV vectors in cancer therapy and cellular reprogramming fields.