Assessment of air pollution genotoxicity by RAPD in Evernia prunastri L. Ach. from around iron-steel factory in Karabuk, Turkey

Cansaran-Duman D., ATAKOL O., Aras S.

JOURNAL OF ENVIRONMENTAL SCIENCES, cilt.23, sa.7, ss.1171-1178, 2011 (SCI-Expanded) identifier identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 23 Sayı: 7
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1016/s1001-0742(10)60505-0
  • Dergi Adı: JOURNAL OF ENVIRONMENTAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1171-1178
  • Anahtar Kelimeler: air pollution, Evernia prunastri, RAPD, genotoxicity, AMPLIFIED POLYMORPHIC DNA, HEAVY-METALS, HYPOGYMNIA-PHYSODES, LICHEN, EXPOSURE, DEPOSITION, ELEMENTS, ASSAY
  • Ankara Üniversitesi Adresli: Evet

Özet

Lichens are widely used in biomonitoring studies of air pollution, either as bioindicators of air quality or as bioaccumulators of atmospheric deposition. Over the past decade, several molecular techniques have been developed to provide information on diversity, genotoxicology, genetic relationships, etc. The heavy metal contents of Evernia prunastri samples were determined by atomic absorption spectrometry. The Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) method was used to describe the pattern of DNA band variation in the samples influenced by the environmental pollution. The study was designed to describe the level of pollution in an area contaminated with smoke and waste from an iron-steel factory, and to reveal the level of potential genotoxic agents around this source of pollution. The study also examined the suitability of the lichen samples for the detection of genotoxicity.