Analysis of genetic diversity among common bean germplasm by start codon targeted (SCoT) markers

YEKEN M. Z., Emiralioglu O., Ciftci V., BAYRAKTAR H., Palacioglu G., ÖZER G.

MOLECULAR BIOLOGY REPORTS, cilt.49, sa.5, ss.3839-3847, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 49 Sayı: 5
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s11033-022-07229-z
  • Dergi Adı: MOLECULAR BIOLOGY REPORTS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.3839-3847
  • Anahtar Kelimeler: Genetic differentiation, Genetic diversity, Genetic resources, Phaseolus breeding, Phaseolus vulgaris, Population structure, PHASEOLUS-VULGARIS L., POPULATION-STRUCTURE, POLYMORPHISM, ACCESSIONS, ORIGIN, SYSTEM, ISSR
  • Ankara Üniversitesi Adresli: Evet

Özet

Background Breeding strategies to improve modern varieties having high yield, high nutritional value and resistance to biotic and abiotic stress, etc. is very important to make up for the food deficiencies. Molecular studies as a tool in breeding programs for the characterization of germplasm have been performed with several DNA marker systems. Materials and methods In the present study, the genetic diversity of 53 common bean landraces and 22 registered varieties from Turkey, and 12 genotypes from USDA was investigated using start codon targeted (SCoT) markers for the first time worldwide. The 8 primers having stronger and more polymorphic bands were used for PCR amplification. Results The mean polymorphic band of all primers was found as 13.13. The average of polymorphic information content and resolving power values was 0.34 and 7.55, respectively. Analysis of molecular variance (AMOVA) explored the existence of higher genetic diversity within populations accounting for 92% compared to among populations variations. According to cluster analysis (UPGMA) and genetic structure based on SCoT data, accessions were separated into Andean (PopA) and Mesoamerican PopB) gene pools. Moreover, accessions were mostly placed in the same groups/subgroups according to their geographical origin. Conclusions A high level of genetic diversity was observed between the investigated accessions in this work. The findings will help to plant breeders to characterize common bean accessions.