Erythropoietin changes contractility, cAMP, and nitrite levels of isolated rat hearts

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Kaygisiz Z., Erkasap N., YAZIHAN N., Sayar K., Ataoglu H., Uyar R., ...Daha Fazla

JOURNAL OF PHYSIOLOGICAL SCIENCES, cilt.56, sa.3, ss.247-251, 2006 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 56 Sayı: 3
  • Basım Tarihi: 2006
  • Doi Numarası: 10.2170/physiolsci.rp004806
  • Dergi Adı: JOURNAL OF PHYSIOLOGICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.247-251
  • Anahtar Kelimeler: erythropoietin, heart, contractility, cAMP, nitrite, RECOMBINANT-HUMAN-ERYTHROPOIETIN, DEPENDENT PROTEIN-KINASE, STAGE RENAL-DISEASE, ENDOTHELIAL-CELLS, CA2+ CURRENT, SARCOPLASMIC-RETICULUM, CARDIAC CONTRACTILITY, HEMODIALYSIS-PATIENTS, OXIDE PRODUCTION, IN-VIVO
  • Ankara Üniversitesi Adresli: Evet

Özet

There is enough evidence that erythropoietin (EPO) may be involved in cardiovascular function. Therefore we have investigated the possible effects of EPO on left ventricular developed pressure, +dP/dt(max), heart rate, tissue cAMP, and nitrite levels. Isolated rat hearts were perfused under constant flow (10 ml/min) conditions with modified Krebs-Henseleit solution and. recombinant human erythropoietin at doses of 100, 200, 500, and 1,000 IU/kg was administered as bolus injections. EPO at 100 IU/kg decreased, but higher doses (500 and 1,000 IU/kg) raised the developed pressure and +dP/dt(max). However, it did not affect heart rate or coronary perfusion pressure when all the respective doses were applied. EPO at 100 IU/kg increased nitrite, and at 1,000 IU/kg it raised cAMP. Our results suggest that EPO may produce dose-dependently negative and positive inotropic effects on myocardial contractility in isolated rat hearts. NO and cAMP may be involved in negative and positive inotropic effects of EPO, respectively.