Akademik Veri Yönetim Sistemi
TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, cilt.28, sa.5, ss.837-843, 2004 (SCI-Expanded)
The effects of different extenders and cryoprotectants on the viability and fertilizing ability of frozen spermatozoa from the mirror carp, Cyprinus carpio L. (1758), were investigated. Semen was collected from anesthesized males by the abdominal massage method. Having determined the main spermatological properties (volume, motility, duration of motility, total spermatozoa number, concentration and pH), the pooled ejaculates were diluted with 3 extenders containing different cryoprotectants (15% DMSO, 15% DMA, 15% Glycerol) individually. One part semen was added to 3 parts extender. The diluted semen was packaged in 0.5 ml straws and left to equilibrate for 45 min at 4 degreesC. Following the equilibration, the straws were exposed to liquid nitrogen vapor for 10 mins and plunged into the liquid nitrogen. Afterwards frozen semen in the straws was thawed in a waterbath at 30 degreesC for 30 s to determine the motility and movement duration with regard to the post-thaw duration.