Optimization study of coumarin 7-hydroxylase enzyme activity in mouse brain and liver

Özdamar E. D., EKE B.

Fabad Journal of Pharmaceutical Sciences, cilt.35, sa.2, ss.77-84, 2010 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 35 Sayı: 2
  • Basım Tarihi: 2010
  • Dergi Adı: Fabad Journal of Pharmaceutical Sciences
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.77-84
  • Anahtar Kelimeler: Brain, Coumarin 7-hydroxylase, CYP450, Liver, Mouse
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study, the optimum conditions (pH, amount of protein, substrate concentration, incubation time) of a cytochrome P450 dependent enzyme, coumarin 7-hydroxylase (COH), activity were determined in microsomal fractions of mouse liver and brain. It.s indicated that different reaction conditions are required for this enzyme activity in mouse liver and brain microsomes. The results obtained, reveal the requirement of optimization studies on COH activity for different tissues of mouse.