Akademik Veri Yönetim Sistemi
Symposium on Prospects for the 3rd Millenium Agriculture, Cluj-Napoca, Romanya, 5 - 06 Ekim 2006, cilt.63, ss.1-4
We have initiated an integrative and quantitative analysis of transcript, protein, and metabolite changes following abiotic stress imposition in an effort to enhance production efficiency under stress conditions and to understand the plant-derived contribution to unproved wine characteristics. In collaboration with the International Grape Genomics Program community (www.vitaceae.org), we have developed the first Affymetrix GeneChip for Vilis vinifera and related hybrids containing probe sets for approximately 16,000 genes, in order to conduct large-scale transcript profiling. In addition, we are using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) coupled with tandem mass spectrometry, and gas chromatography-mass spectrometry (GC-MS) to conduct parallel, large-scale protein and metabolite profiles. Custom bioinformatics tools and resources are being developed to integrate experimental results and information about transcript, protein, and metabolite changes that arise in response to abiotic stress exposure. Results from transcript, protein and metabolite profiling from abiotically-stressed leaves from a long-term experiment are summarized. These analyses will be used to elucidate specific metabolic pathways that are perturbed by water deficit and salinity treatments. The comprehensive data sets generated by this project will be critical for our ability to consider engineering strategies for improving plant stress tolerance in the future.