Sensitive Nucleic Acid Detection at NH2-MWCNTs Modified Glassy Carbon Electrode and its Application for Monitoring of Gemcitabine-DNA Interaction

Shahzad S., KARADURMUŞ L., DOĞAN TOPAL B., TAŞKIN TOK T., Shah A., Ozkan S. A.

Electroanalysis, cilt.32, sa.5, ss.912-922, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Sayı: 5
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1002/elan.201900597
  • Dergi Adı: Electroanalysis
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Applied Science & Technology Source, BIOSIS, Chimica, Communication Abstracts, Metadex, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.912-922
  • Anahtar Kelimeler: Functionalized nanomaterials, Biosensor, dsDNA, Groove binding, Gemcitabine, in silico methods, MOLECULAR-DYNAMICS, NANOTUBES, BIOSENSOR, IDENTIFICATION, DOCKING, SINGLE, DRUG
  • Ankara Üniversitesi Adresli: Evet

Özet

© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, WeinheimAn electrochemical dsDNA nanobiosensor was fabricated using amino-functionalized multi walled carbon nanotubes modified glassy carbon electrode (NH2fMWCNTs/GCE) for the sensitive detection of DNA bases and electrochemical monitoring of drug-DNA interaction. The influence of functional groups on MWCNT was studied by MWCNT functionalized with NH2 (NH2fMWCNTs) and COOH (COOHfMWCNT) groups based on the signal of DNA bases. The modified electrodes were characterized by scanning electron microscopy. One layer of calf thymus double stranded deoxyribonucleic acid (ct-dsDNA) was immobilized onto the NH2fMWCNTs/GCE (dsDNA/NH2fMWCNTs/GCE). The dsDNA/NH2fMWCNTs/GCE were used to investigate the interaction between the dsDNA and the anticancer drug gemcitabine by differential pulse voltammetry in acetate buffer of pH 4.70. For the confirmation of interaction, the lowering in intensity of the current signals of guanine and adenine was considered as an indicator. Electrochemical impedance spectroscopy studies were performed for the comparison of the modified surfaces. In order to define and visualize the interaction mechanism between gemcitabine and dsDNA/NH2fMWCNTs/GCE at the molecular level, in silico methods including docking and molecular dynamics simulations were employed.