A comparative study on the in vitro cytotoxic responses of two mammalian cell types to fullerenes, carbon nanotubes and iron oxide nanoparticles

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Dönmez Güngüneş Ç., Seker Ş., Elçin A. E., Elçin Y. M.

DRUG AND CHEMICAL TOXICOLOGY, cilt.40, sa.2, ss.215-227, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 40 Sayı: 2
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1080/01480545.2016.1199563
  • Dergi Adı: DRUG AND CHEMICAL TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.215-227
  • Anahtar Kelimeler: Nanoparticles, cytotoxicity, iron (II, III) oxide, carbon nanotube, fullerene, in vitro toxicity, impedance based assay, MAGNETITE NANOPARTICLES, DELIVERY, SURFACE, SKIN, VIVO, DOXORUBICIN, ANTIOXIDANT, COMPOSITES, POLYMERS, EFFICACY
  • Ankara Üniversitesi Adresli: Evet

Özet

The present study was designed to evaluate and compare the time- and dose-dependent cellular response of human periodontal ligament fibroblasts (hPDLFs), and mouse dermal fibroblasts (mDFs) to three different types of nanoparticles (NPs); fullerenes (C-60), single walled carbon nanotubes (SWCNTs) and iron (II, III) oxide (Fe3O4) nanoparticles via in vitro toxicity methods, and impedance based biosensor system. NPs were characterized according to their morphology, structure, surface area, particle size distribution and zeta potential by using transmission electron microscopy, X-ray diffraction, Brunauer-Emmett-Teller, dynamic light scattering and zeta sizer analyses. The Mossbauer spectroscopy was used in order to magnetically characterize the Fe3O4 NPs. The hPDLFs and mDFs were exposed to different concentrations of the NPs (0.1, 1, 10, 50 and 100 mg/mL) for predetermined time intervals (6, 24 and 48 h) under controlled conditions. Subsequently, NP exposed cells were tested for viability, membrane leakage and generation of intracellular reactive oxygen species. Additional to in vitro cytotoxicity assays, the cellular responses to selected NPs were determined in real time using an impedance based biosensor system. Taken together, information obtained from all experiments suggests that toxicity of the selected NPs is cell type, concentration and time dependent.