Biochemical Basis of New Generation Nucleotide Sequencing Methods

AKYOL İ., YILDIZ M. A., Tutar E.

KAHRAMANMARAS SUTCU IMAM UNIVERSITY JOURNAL OF NATURAL SCIENCES, cilt.20, sa.1, ss.1-15, 2017 (ESCI) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20 Sayı: 1
  • Basım Tarihi: 2017
  • Doi Numarası: 10.18016/ksujns.76089
  • Dergi Adı: KAHRAMANMARAS SUTCU IMAM UNIVERSITY JOURNAL OF NATURAL SCIENCES
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.1-15
  • Anahtar Kelimeler: emulsion PCR, pyrosequencing, reversible terminator sequencing, ligation sequencing, nanopore sequencing, semiconductor sequencing, PHI29 DNA-POLYMERASE, PYROSEQUENCING TECHNOLOGY, REVERSIBLE TERMINATORS, POLYNUCLEOTIDE MOLECULES, NANOPORE, DISCRIMINATION, PYROPHOSPHATE, TRIPHOSPHATES, CHALLENGES, PRIMERS
  • Ankara Üniversitesi Adresli: Evet

Özet

Sanger and Maxam-Gilbert sequencing methods are pronounced as the first generation sequencing. Chain termination sequencing approach is widely used and the first human genome project was completed by this method. However, this method has certain limitations especially in terms of speed, ease of use and cost. New generation sequencing approaches are important thanks to their capacity to work with many parallels, high efficiency and low cost. In this review, emulsion PCR approach, which is required for the amplification of template DNA so that it can produce sufficient amount of emission on the next generation sequencing, is described. Moreover, biochemical basis of the new generation sequencing approaches; pyro sequencing, reversible terminator sequencing, ligation sequencing, nanopore sequencing and semiconductor sequencing, are also described.