Research Information System
Biointerface Research in Applied Chemistry, vol.12, no.6, pp.8205-8218, 2022 (ESCI)
Abstract: This study aimed to investigate the effect of sustained-release vitamin D loaded in poly-lactic
acid (PLA) nanoparticles and applied to grafting materials around titanium (ti) dental implants.
A total of 48 implants were inserted into the standardized bone defects created in the iliac crest of 6
sheep, divided into 4 different experimental sites (empty control, autografted, xenografted, and
autograft + xenografted). Vitamin D3, which was encapsulated by PLA nanoparticles, was prepared
and applied in half of the defects and left to heal for 3 and 6 weeks. New bone formation (NBF%) and
bone-implant contact (BIC%) values were evaluated by histologic and histomorphometric analyses (P
< 0.05). Nanoparticles with a yield of 80.30% ± 2.14% and high encapsulation efficiency of 72.99% ±
2.20% were achieved. In all sections, osseointegration was observed, with no signs of inflammation,
necrosis, or foreign body reaction. NBF% and BIC% were significantly higher in vitamin D3–loaded
groups (P < 0.001). In both healing periods, the highest NBF% and BIC% have been recorded in the
autograft + xenograft groups. (For the respective third and sixth weeks: the NBF% was 49.63 ± 1.53
and 74.25 ± 0.96, and BIC% was 54.26 ± 0.66 and 82.59 ± 2.09, respectively; P < 0.001). Sustainedrelease
of vitamin D loaded into the PLA system demonstrated high biocompatibility, favorable
sustained release of vitamin D3, and improved NBF% and BIC% around ti implants placed in boxshaped
sheep iliac crest defects. Further investigations are required for their clinical applicability.
Keywords: autograft; dental implant; osseointegration; poly-lactic acid; sustained release; vitamin D3;
xenograft.